Difference of Cationic Amino Acid Transporters mRNA Expression in Different Intestinal Segments of Chicken

The objective of this study was to investigate the difference of mRNA expression of cationic amino acid transporter rBAT(system b0,+),y+LAT2(system y+L),CAT1(system y+)and CAT4(system y+) in different intestinal segments of chicken by relative quantitative RT-PCR.Yellow covered chickens at age of 30 days were used and the different intestinal segments were collected.The result showed: rBAT and y+LAT2 mRNA abundance in colorectum were significant lower than that of duodenum,jejunum and ileum(P0.01).rBAT and y+LAT2 mRNA expression in ileum were higher than in duodenum and jejunum,but had no significant difference among them(P0.05);The expression of CAT1 mRNA in colorectum was very significantly higher than that of duodenum,jejunum and ileum(P0.01).Expression abundance in ileum was higher than in jejunum significantly(P0.01) and higher than duodenum by 27.9 %(P=0.111).Same as CAT1 mRNA,the expression of CAT4 mRNA in colorectum was higher than that of duodenum,jejunum and ileum significantly(P0.01);There was no difference among duodenum,jejunum and ileum(P0.05).The result indicates that the tissue-specific of transporter mRNA expression of cationic amino acid transporter system b0,+ and system y+LAT2 are similar and discriminated with that of system y+.