Objective To investigate the role of echocardiography in the evaluation of acclimatization at high altitude.Methods The physiologic indexes populations exposed to high altitude for one week,two months,more than 1 year and re-exposed populations were collected and underwent echocardiographical examinations.The pulse rate,respiration and hemoglobin were used to evaluate acclimatization levels.Results The acclimation rate in the four groups was 43.06%,57.41%,79.49% and 56.25% respectively.There were five high altitude polycythemia volunteers in the re-exposed group.The left ventricular internal diameter was significantly different between acclimated and un-acclimated groups.Cardiac indexes were also significantly different between the initial,entire acclimatization and re-exposed populations.The area under the curve was 0.766 in the assessment of acclimatization by cardiac indexes.Conclusion Cardiac functions play a role in the process of acclimatization.Cardiac indexes are of great value for evaluating acclimatization,especially long-term migrations.