Effect of Sini decoction on expression of caveolin-1 and eNOS in EAhy926 cells injured by homocysteine

AIM: To investigate the mechanism of Sini decoction in treating human vascular endothelial cell injury and the roles of caveolin-1 and nitric oxide(NO) system in this procedure.METHODS: Model of human umbilical vein endothelial EAhy926 cells injured by homocysteine(Hcy) was established.The protective effect of Sini decoction on the injured EAhy926 cells was observed,and the expression of caveolin-1 and endothelial nitric oxide synthase(eNOS) was detected by real-time fluorescence quantitative PCR and Western blotting.RESULTS: Compared with control group,the Hcy-treated EAhy926 cells showed reduced adherent cell number and NO concentration in culture supernatant,decreased expression of eNOS mRNA and protein,and increased expression of caveolin-1 mRNA and protein(all P 0.05).Compared with Hcy group,better growth of adherent cells,elevated NO concentration in culture supernatant,attenuated expression of caveolin-1 mRNA and protein,and enhanced expression of eNOS mRNA and protein in Sini decoction groups were observed(all P 0.05).CONCLUSION: Homocysteine may injure EAhy926 cells by enhancing the expression of caveolin-1 and suppressing the expression of eNOS,while Sini decoction may protect EAhy926 cells by suppressing the expression of caveolin-1 and enhancing the expression of eNOS.