Cloning Indification and Sequence Analysis of the a Subunit Gene of SLT-IIe Toxin

The 987 bp nucleotide fragment including the whole encoding regions of slt IIeA was amplified by PCR from a E.coli strain called ED1which responsible for the edema disease in piglets in Hubei province and the 987bp nucleotide fragment.was cloned into pMD 18T vector. The 1029 nucleotides sequence including the whole encoding regions of slt IIeA between site BamHⅠ and site HindIII were analyzed and the amino acids were deduced .The nucleotides sequence of whole encoding region and deduced amino acids were compared between strain ED1 and strain S1179 reported by foreigner. Result showed that the nucleotides sequence homology between ED1 and S1179 was 99.6% and amino acids homology was 99.7%. It provided an fundmental basis for the further studying on biological characterization of slt IIeA, molecular diagnosis and protection of edema disease.