[Objective] The scientific basis for the rational development of black kerneled rice was provided through the experiment.[Method] The extraction of anthocyanin from black kerneled rice was studied with the orthogonal test of single-factor.[Results] The best extracting condition of anthocyanin was determined: ethanol solution,75%,ratio of liquid to raw material,1∶8;extraction temperature,30 ℃;extracting time,30 min;and the extraction rate of anthocyanin was up to 94.4% after twice time extraction under the condition.[Conclusion] The processing condition resulted in the good extracting efficiency of anthocyanin from black kerneled rice and low cost.