同位素
化学
氘
动力学同位素效应
烷基苯
同位素
质谱法
色谱法
气相色谱法
化学极性
分析化学(期刊)
有机化学
偶极子
分子
催化作用
物理
量子力学
作者
Nimisha Thakur,Saba Aslani,Daniel W. Armstrong
标识
DOI:10.1016/j.aca.2021.338490
摘要
The separation of deuterated compounds from their protiated counterparts is essential in areas of drug discovery and development, investigating kinetic isotope effects and quantitative methods of non-mass spectrometry-based stable isotope dilution assay (non-MS SIDA). The separations of 47 isotopologue pairs of common compounds and drugs were achieved by gas-liquid chromatography, employing twelve different stationary phases. Polydimethylsiloxane phase, phenyl substituted polydimethylsiloxane phases, wax phases, ionic liquid phases, and chiral stationary phases were selected to encompass a wide polarity range and diverse chemical interactions. The best-performing stationary phases are presented for separation of protic-polar, aprotic-dipolar, nonpolar analytes. Overall, the IL111i, SPB-20, and PAG stationary phases were remarkable in their ability to separate the isotopologues. The isotope effect was also evaluated. It was observed that nonpolar stationary phases often exhibit an inverse isotope effect in which heavier isotopic compounds elute earlier than their lighter counterparts. Conversely, polar stationary phases often show a normal isotope effect, while those of intermediate polarities can show both effects depending on the isotopologues. The location of deuterium atoms, however, affects isotopologue retention times. Deuterium substituted aliphatic groups appear to have a greater inverse isotope effect on retention than aromatic substituents.
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