Rapid start-up of autotrophic shortcut nitrification system in SBR and microbial community analysis

Shortcut nitrification is crucial for application of autotrophic nitrogen removal which is beneficial for treating carbon-limited wastewater. In this experiment, rapid start-up of autotrophic shortcut nitrification system was studied in a small sequencing batch reactor (SBR) built in laboratory with intermittent aeration operation mode. The influent was artificially simulated inorganic domestic wastewater (the ammonium nitrogen concentration was 35.19-57.54 mg/L), the pH value was 7.6-7.8, the hydraulic loading was 1L, the operating temperature was 24.3-28.3 °C, and the dissolved oxygen (DO) was 2-4 mg/L and 0.5-0.9 mg/L at the stage of complete nitrification sludge domestication and shortcut nitrification sludge domestication. High-throughput sequencing technology was used to analyse the composition and changes of microbial populations in sludge. The experimental results showed that on the 24th day of the experiment, shortcut nitrification was started successfully, the accumulation rate of nitrite was 81.63% and the removal efficiency of ammonium nitrogen was 99.25%; the richness of the main denitrifying bacteria phylum Proteobacteria increased from 30.21% to 42.85%; the richness of Nitrosomonas (ammonia oxidizing bacteria, AOB) increased from 0.37% to 22.43%, and at the species level, AOB was the salt-tolerant bacteria Nitrosomonas. europaea; the richness of Nitrospira (nitrite oxidizing bacteria, NOB) decreased from 2.59% to 0.47%.