基质(水族馆)
生物催化
酶
蛋白质工程
背景(考古学)
超分子化学
对映选择合成
组合化学
化学
催化效率
滥交
材料科学
纳米技术
催化作用
有机化学
生物
分子
离子液体
古生物学
生态学
作者
Carolina I. Giunta,Isabel Cea‐Rama,Sandra Alonso,Manon L. Briand,Rafael Bargiela,Cristina Coscolín,Philippe F.-X. Corvini,Manuel Ferrer,J. Sanz‐Aparicio,Patrick Shahgaldian
出处
期刊:ACS Nano
[American Chemical Society]
日期:2020-12-11
卷期号:14 (12): 17652-17664
被引量:19
标识
DOI:10.1021/acsnano.0c08716
摘要
Owing to their outstanding catalytic properties, enzymes represent powerful tools for carrying out a wide range of (bio)chemical transformations with high proficiency. In this context, enzymes with high biocatalytic promiscuity are somewhat neglected. Here, we demonstrate that a meticulous modification of a synthetic shell that surrounds an immobilized enzyme possessing broad substrate specificity allows the resulting nanobiocatalyst to be endowed with enantioselective properties while maintaining a high level of substrate promiscuity. Our results show that control of the enzyme nano-environment enables tuning of both substrate specificity and enantioselectivity. Further, we demonstrate that our strategy of enzyme supramolecular engineering allows the enzyme to be endowed with markedly enhanced stability in an organic solvent (i.e., acetonitrile). The versatility of the method was assessed with two additional substrate-promiscuous and structurally different enzymes, for which improvements in enantioselectivity and stability were confirmed. We expect this method to promote the use of supramolecularly engineered promiscuous enzymes in industrially relevant biocatalytic processes.
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