克拉斯
生物
癌变
近交系
肺
肺癌
转基因小鼠
癌症研究
转基因
癌症
免疫学
遗传学
突变
病理
内科学
基因
医学
作者
Francesca Sozio,Tiziana Schioppa,Silvano Sozzani,Annalisa Del Prete
标识
DOI:10.1016/bs.mcb.2020.09.005
摘要
Chemical induced carcinogenesis together with genetically engineered mouse models represent important approaches for the study of the complex mechanisms involving genotype and environmental factors in cancer development, including lung cancer. The induction of lung tumor in mice with urethane (ethyl carbamate) is considered a valuable model of Kras-driven lung cancer. However, inbred mouse strains show variable susceptibility to lung tumor formation, with C57BL/6 background, widely used to study many transgenic and null mutations, highly resistant to lung carcinogenesis. Here is described a protocol of urethane-induced lung cancer effective in lung tumor induction in C57BL/6J strain. Multiple urethane injections are needed to overcome genetic resistance and induce in a reproducible manner lung carcinogenesis in C57BL/6J background mice.
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