荧光
碳量子点
大肠杆菌
检出限
适体
橙色(颜色)
化学
量子点
橙汁
纳米颗粒
碳纤维
光化学
核化学
分析化学(期刊)
食品科学
色谱法
材料科学
纳米技术
生物化学
复合数
生物
光学
分子生物学
物理
基因
复合材料
作者
Xuetao Hu,Yanxiao Li,Yanhua Xu,Ziyu Gan,Xiaobo Zhang,Jiyong Shi,Xiaowei Huang,Zhihua Li,Yahui Li
出处
期刊:Food Chemistry
[Elsevier]
日期:2021-03-01
卷期号:339: 127775-127775
被引量:132
标识
DOI:10.1016/j.foodchem.2020.127775
摘要
Carbon quantum dots (CQDs) prepared by a green one-step approach was used for sensitive and selective assay of Escherichia coli O157: H7 (E. coli). CQDs was synthesized from orange peel as a carbon source via a microwave-assisted method. The CQDs displayed strong green fluorescence under excitation wavelength of 420 nm. A fluorescent probe (CQDs-MNPs) for E. coli was fabricated based on CQDs labeled with aptamer (aptamer-CQDs) and magnetic nanoparticles labeled with complementary DNA (cDNA-MNPs). Fluorescent intensity of the CQDs-MNPs was decreased with addition of E. coli. The linearity between fluorescent intensity and E. coli concentration was used for developing a fluorescent method with detecting range of 500–106 CFU/mL and detection limit of 487 CFU/mL. Milk samples contaminated by E. coli were analyzed by this method, and the results agreed with that achieved by plate-counting methods. This fluorescent probe exhibits great potential in guaranteeing food quality and safety.
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