ANTITUMOR EFFECTS OF L-CITRULLINE ON HELA OVER CANCER CELL LINES.

Ovarian cancer is the deadliest gynecological malignancy. The aim of this study is to examine the anticancer effects of L-Citrulline in HeLa cell culture.HeLa cells were cultured in complete Eagle's Minimum Essential Medium. HeLa cells were seeded in 96 well plates and incubated with the L-Citrulline. After incubation MTT dye was added and incubated. Annexin-V technique was used to test the apoptosis. The activated Caspases of HeLa cells by L-Citrulline exposure were measured with the Caspase 3/7 technique. For statistical analysis, One-way variance analysis of GraphPad Prism 6.0 for Windows was used.L-Citrulline showed its toxicity on HeLa cells in dose dependent manner in application times of 24 and 48 hours. IC50 dose of L-Citrulline is 0.19 and 0.16 mg/mL for 24 and 48 hours respectively. HeLa cells exposed to IC50 dose of L-Citrulline for 24 hours, the percentages of dead, early apoptotic and late apoptotic cells were detected to be 0.75%, 23.05% and 12.75% respectively. The differences of wideness of scratch area were observed at initial stage and after 24 hours of the application of L-Citrulline.L-Citrulline showed its toxicity on HeLa cells in dose dependent manner. Based on Annexin and Caspase findings it can be concluded that L-Citrulline is proapoptotic on HeLa cells in short time of exposure. L-Citrulline also showed migration inhibitory effect. Findings of this study can be concluded that L-Citrulline worth to be investigated for its anticancer activities in vitro and in vivo as a candidate for cancer therapy agent.