Facile fabrication of tyrosine-functionalized hypercrosslinked polymer for sensitive determination of nitroimidazole antibiotics in honey and chicken muscle

The preparation of tyrosine-functional novel hypercrosslinked polymer (HCP@Tyr) was achieved by knitting 1,3,5-triphenylbenzene with l-tyrosine via simple Friedel-Crafts reaction. The HCP@Tyr displayed large surface area (1220 m2 g-1) and high adsorption capacity (30 mg g-1) for nitroimidazoles (NDZs). With HCP@Tyr as potent sorbent, an efficient extraction method was developed to simultaneously extract five NDZs from honey and chicken muscle samples before high performance liquid chromatography-diode array detection. Under optimized conditions, the proposed method provided a good linearity in the range of 0.75-200.0 ng g-1 for honey and 1.5-100.0 ng g-1 for chicken muscle sample. Low detection limits (S/N = 3) were obtained to be 0.10-0.24 ng g-1 for honey and 0.15-0.30 ng g-1 for chicken muscle. The method recovery was 80.7-120% with relative standard deviations below 9.2%. The HCP@Tyr based method can serve as a reliable and sensitive tool for detection of NDZs in honey and chicken muscle samples.