莫里斯水上航行任务
肌醇
磷酸蛋白质组学
磷脂酰肌醇
磷酸化
组织蛋白酶B
蛋白质组
海马体
肽
药理学
生物
化学
生物化学
细胞生物学
蛋白质磷酸化
神经科学
蛋白激酶A
酶
受体
作者
Mingxian Geng,Fanrui Zhao,Huiqi Lu,Fang Li,Ji Wang,Chunlei Liu,Weihong Min
标识
DOI:10.1016/j.foodres.2022.111311
摘要
This work aimed to explore the underlying mechanisms of memory improvement effects of a walnut derived peptide WNP-10. The morris water maze test, combined with ultrastructural observation, hematoxylin and eosin and Nissl staining showed that WNP-10 significantly improved the learning and memory capability of the scopolamine-injured mice. The four-dimensional label-free quantification proteomics analysis identified 88 differentially expressed proteins in the WNP-10-treated group compared with scopolamine-induced impairment group. Pathway enrichment analysis and western blotting demonstrated that the WNP-10 can regulate the phosphatidylinositol-3-phosphate 5-kinase, cathepsin L, N-acetylgalactosamine 6-sulfate sulfatase and AP-3 complex subunit mu-1 expression to affect inositol phosphate metabolism, thereby maintaining lysosome homeostasis in scopolamine-injured mice. Notably, the results of phosphoproteomics demonstrated that WNP-10 administration resulted in the increased phosphorylation of phosphatidylinositol-3-phosphate 5-kinase. These findings provide novel insights into the underlying mechanism of memory improvement of walnut peptides.
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