校准曲线
校准
啤酒-兰伯特定律
样品(材料)
分析物
直线(几何图形)
吸光度
阅读(过程)
系统误差
计算机科学
法学
统计
数学
光学
物理
化学
色谱法
几何学
政治学
检出限
摘要
Calibration curves allow instrument calibration by predicting the concentration of an analyte in a sample from the reading of the instrument. This curve is constructed as the regression straight line that best fits the relationship between some known concentration standards and their respective instrument readings. An example is the Beer-Lambert Law, used to predict the concentration of a new sample from its absorbance obtained by spectrometry. The issue is that usually this methodology is misapplied. In this paper, we want to clarify this point, explaining what the error consists of and how (easily) to fix it, with the intention of ensuring that it does not continue to be reproduced in the experimental scientific work.
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