生物
磷酸化
钙调蛋白
细胞生物学
激酶
拟南芥
钙
蛋白质磷酸化
第二信使系统
钙信号传导
信号转导
转录因子
蛋白激酶A
钙结合蛋白
生物化学
突变体
基因
化学
有机化学
酶
作者
Lifan Sun,Jun Qin,Wu Xiaoyun,Jinghan Zhang,Jie Zhang
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2022-07-21
卷期号:34 (10): 4088-4104
被引量:3
标识
DOI:10.1093/plcell/koac209
摘要
Plants utilize localized cell-surface and intracellular receptors to sense microbes and activate the influx of calcium, which serves as an important second messenger in eukaryotes to regulate cellular responses. However, the mechanisms through which plants decipher calcium influx to activate immune responses remain largely unknown. Here, we show that pathogen-associated molecular patterns (PAMPs) trigger calcium-dependent phosphorylation of CAM-BINDING PROTEIN 60-LIKE G (CBP60g) in Arabidopsis (Arabidopsis thaliana). CALCIUM-DEPENDENT PROTEIN KINASE5 (CPK5) phosphorylates CBP60g directly, thereby enhancing its transcription factor activity. TOUCH 3 (TCH3) and its homologs CALMODULIN (CAM) 1/4/6 and CPK4/5/6/11 are required for PAMP-induced CBP60g phosphorylation. TCH3 interferes with the auto-inhibitory region of CPK5 and promotes CPK5-mediated CBP60g phosphorylation. Furthermore, CPKs-mediated CBP60g phosphorylation positively regulates plant resistance to soil-borne fungal pathogens. These lines of evidence uncover a novel calcium signal decoding mechanism during plant immunity through which TCH3 relieves auto-inhibition of CPK5 to phosphorylate and activate CBP60g. The findings reveal cooperative interconnections between different types of calcium sensors in eukaryotes.
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