生物
聚糖
丁酸盐
拟杆菌
人口
机制(生物学)
计算生物学
遗传学
生物化学
细菌
发酵
糖蛋白
认识论
哲学
社会学
人口学
作者
Jun Feng,Yili Qian,Zhichao Zhou,Sarah Ertmer,Eugenio I. Vivas,Freeman Lan,Joshua J. Hamilton,Federico E. Rey,Karthik Anantharaman,Ophelia S. Venturelli
标识
DOI:10.1016/j.chom.2021.12.006
摘要
Polysaccharide utilization loci (PULs) are co-regulated bacterial genes that sense nutrients and enable glycan digestion. Human gut microbiome members, notably Bacteroides, contain numerous PULs that enable glycan utilization and shape ecological dynamics. To investigate the role of PULs on fitness and inter-species interactions, we develop a CRISPR-based genome editing tool to study 23 PULs in Bacteroides uniformis (BU). BU PULs show distinct glycan-degrading functions and transcriptional coordination that enables the population to adapt upon loss of other PULs. Exploiting a BU mutant barcoding strategy, we demonstrate that in vitro fitness and BU colonization in the murine gut are enhanced by deletion of specific PULs and modulated by glycan availability. PULs mediate glycan-dependent interactions with butyrate producers that depend on the degradation mechanism and glycan utilization ability of the butyrate producer. Thus, PULs determine community dynamics and butyrate production and provide a selective advantage or disadvantage depending on the nutritional landscape.
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