血栓弹性测定
纤维蛋白原
纤维蛋白
凝结
因素十三
化学
凝血酶
白蛋白
凝血酶原复合物浓缩物
凝血因子
凝血时间
血小板
色谱法
生物化学
免疫学
医学
内科学
华法林
心房颤动
作者
Tobias Koller,Nadia Kinast,Andres Guilarte Castellanos,Sergio Perez Garcia,Pilar Paniagua Iglesias,Xavi León Vintro,José Mateo,Noelia Vilalta Setó,Ma. Victòria Moral García,Ana Belén Moreno-Castaño,J Aznar-Salatti,Ginés Escolar Albaladejo,Maribel Díaz‐Ricart
标识
DOI:10.1186/s13049-021-00867-5
摘要
Abstract Background Colloid fluids supplemented with adequate combinations of coagulation factor concentrates with the capability to restore coagulation could be a desirable future treatment component in massive transfusion. Methods Starting from a coagulation factor and blood cell-free albumin solution we added Prothrombin Complex Concentrate, Fibrinogen Concentrate and Factor XIII in different combinations and concentrations to analyze their properties to restore thromboelastometry parameters without the use of plasma. Further analysis under the presence of platelets was performed for comparability to whole blood conditions. Results Albumin solutions enriched with Fibrinogen Concentrate, Factor XIII and Prothrombin Complex Concentrate at optimized concentrations show restoring coagulation potential. Prothrombin Complex Concentrate showed sufficient thrombin formation for inducing fibrinogen polymerization. The combination of Prothrombin Complex Concentrate and Fibrinogen Concentrate led to the formation of a stable in vitro fibrin clot. Fibrinogen and Factor XIII showed excellent capacity to improve fibrin clot firmness expressed as Amplitude at 10 min and Maximal Clot Firmness. Fibrinogen alone, or in combination with Factor XIII, was able to restore normal Amplitude at 10 min and Maximal Clot Firmness values. In the presence of platelets, the thromboelastometry surrogate parameter for thrombin generation (Clotting Time) improves and normalizes when compared to whole blood. Conclusions Combinations of coagulation factor concentrates suspended in albumin solutions can restore thromboelastometry parameters in the absence of plasma. This kind of artificial colloid fluids with coagulation-restoring characteristics might offer new treatment alternatives for massive transfusion. Trial registration Study registered at the institutional ethic committee “Institut de Recerca, Hospital Santa Creu i Sant Pau, with protocol number IIBSP-CFC-2013-165.
科研通智能强力驱动
Strongly Powered by AbleSci AI