Transport behavior of baicalin in PC12 cells

Aim To investigate the transport behavior of baicalin in PC12 cells and to explain the material basement of nervous system protection activity of baicalin.Methods PC12 cells were used as for research,and HPLC and MS were applied to detect the transport behavior of baicalin.Results When cells were treated by baicalin of 0.1 mg·L-1,the concentration of baicalin in cells reached a peak when incubating for 30 min and there was no baicalin left after 8 h;when the dosage of baicalin from 0.05 mg·L-1 to 0.4 mg·L-1 was in administration,intracellular baicalin concentration and administration dosage showed linear positive correlation;verapamil and nimodipine had effect on transport;in addition,baicalein and 6-methoxy-baicalin could be detected in the cells.Conclusion Baicalin can transport into PC12 cells and can be affected by some inhibitors,producing compounds.