化学
肽
荧光素酶
小分子
组合化学
生物化学
蛋白质-蛋白质相互作用
生物物理学
配体(生物化学)
化学生物学
细胞生物学
作者
Tsuyoshi Takahashi,Hiroaki Hagiwara
摘要
We report a method for detecting ligand-protein interactions occurring within cells using short peptide reactive tags appended to ligands and proteins, along with a split NanoLuc luciferase. This method can be applied to estimate the binding affinities of ligand-protein interactions and to detect the interactions of proteins with unstable synthetic ligands inside the cells.
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