Microscopic analysis of fragments of destroyed hollow glass microspheres was used to determine the thickness of their walls. It is revealed that the wall thickness of industrial microspheres can vary significantly even within a single glass microsphere. It is shown that as the diameter of hollow glass microspheres increases, the relative wall thickness decreases, and the degree of their heterogeneity increases. The wall thickness variability leads to a significant decrease in the level of critical pressure, which causes the destruction of microspheres, compared to the calculated one.