对氧磷
硫代乙酰胆碱
乙酰胆碱酯酶
吸光度
化学
辣根过氧化物酶
检出限
比色法
阿切
胆碱氧化酶
色谱法
酶
分解
核化学
有机化学
作者
Xu Yan,Yang Song,Xiaoli Wu,Chengzhou Zhu,Xingguang Su,Dan Du
出处
期刊:Nanoscale
[The Royal Society of Chemistry]
日期:2017-01-01
卷期号:9 (6): 2317-2323
被引量:191
摘要
In the present study, a novel colorimetric sensing platform was constructed for quantitative detection of acetylcholinesterase (AChE) activity and its inhibitor. Manganese dioxide (MnO2) nanosheets as an oxidase-mimicking nanomaterial could directly oxidize 3,3',5,5'-tetramethylbenzidine (TMB) into oxTMB without the need for horseradish peroxidase and H2O2. When AChE was introduced, acetylthiocholine could be catalytically hydrolyzed to produce thiocholine, which easily triggers the decomposition of MnO2 nanosheets, causing the decrease of solution absorbance. Owing to the inhibition effect of organophosphorus pesticides, the enzymatic activity was suppressed, preventing the decomposition of MnO2 and resulting in the increase of absorbance. Under optimal conditions, the colorimetric platform shows sensitive responses to AChE and paraoxon in the range of 0.1-15 mU mL-1 and 0.001-0.1 μg mL-1, respectively. The detection limits of AChE and paraoxon reached 35 μU mL-1 and 1.0 ng mL-1, respectively. Furthermore, the MnO2-TMB platform has been used to fabricate test strips for rapid and convenient visual detection of AChE and its inhibitor with highly promising performance.
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