Monitoring of Toxigenic Cyanobacteria Using Next‐Generation Sequencing Techniques

With fast development and wide applications of next generation sequencing (NGS) technologies, pyrosequencing of PCR-amplified fragments that target variable regions within the 16S rRNA gene has quickly become a powerful method for analyzing the microbial community profiles. In this chapter we describe an approach for sequencing 16S rRNA gene amplicons for monitoring of (toxigenic) cyanobacteria and other prokaryotes in environmental samples. Our SOP is particular adapted for relative long amplicon size (~800bp) in order to achieve more accurate taxonomy assignment.