Electrotransformation of Xanthobacter autotrophicus GJ10 and other Xanthobacter strains

This paper describes the development of an electroporation procedure for Xanthobacter autotrophicus GJ10. Electroporation has not previously been described for this species. The procedure was optimized with respect to field strength, pulse time and DNA and cell concentration. High transformation efficiencies of up to 2×106 cells transformed per μg DNA were found. The conditions optimal for the X. autotrophicus GJ10 were used to electrotransform 9 other Xanthobacter strains 4 of which were transformed with high efficiencies. The electrotransformation procedure described here can be of great help for the genetic availability of Xanthobacter species.