生物
精子
细胞外
去极化
鞭毛
细胞生物学
生物物理学
细胞内
顶体反应
细胞内pH值
精子活力
细胞质
生物化学
遗传学
基因
作者
Polina V. Lishko,Inna L. Botchkina,Andriy Fedorenko,Yuriy Kirichok
出处
期刊:Cell
[Elsevier]
日期:2010-02-01
卷期号:140 (3): 327-337
被引量:331
标识
DOI:10.1016/j.cell.2009.12.053
摘要
Human spermatozoa are quiescent in the male reproductive system and must undergo activation once introduced into the female reproductive tract. This process is known to require alkalinization of sperm cytoplasm, but the mechanism responsible for transmembrane proton extrusion has remained unknown because of the inability to measure membrane conductance in human sperm. Here, by successfully patch clamping human spermatozoa, we show that proton channel Hv1 is their dominant proton conductance. Hv1 is confined to the principal piece of the sperm flagellum, where it is expressed at unusually high density. Robust flagellar Hv1-dependent proton conductance is activated by membrane depolarization, an alkaline extracellular environment, endocannabinoid anandamide, and removal of extracellular zinc, a potent Hv1 blocker. Hv1 allows only outward transport of protons and is therefore dedicated to inducing intracellular alkalinization and activating spermatozoa. The importance of Hv1 for sperm activation makes it an attractive target for controlling male fertility.
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