干细胞
移植
体内
脂肪组织
医学
干细胞疗法
病理
正电子发射断层摄影术
细胞
癌症研究
核医学
内科学
化学
生物
细胞生物学
生物化学
生物技术
作者
Min Hwan Kim,Sang‐Keun Woo,Kyo Chul Lee,Gwang Il An,Darpan N. Pandya,Noh Won Park,Sang‐Soep Nahm,Kidong Eom,Kwang Il Kim,Yong Jin Lee,Chan Wha Kim,Joo Hyun Kang,Jeongsoo Yoo,Yong Jin Lee
标识
DOI:10.1016/j.bbrc.2014.11.019
摘要
This study aims to monitor how the change of cell survival of transplanted adipose-derived stem cells (ADSCs) responds to myocardial infarction (MI) via the hexadecyl-4-124I-iodobenzoate (124I-HIB) mediated direct labeling method in vivo. Stem cells have shown the potential to improve cardiac function after MI. However, monitoring of the fate of transplanted stem cells at target sites is still unclear. Rat ADSCs were labeled with 124I-HIB, and radiolabeled ADSCs were transplanted into the myocardium of normal and MI model. In the group of 124I-HIB-labeled ADSC transplantation, in vivo imaging was performed using small-animal positron emission tomography (PET)/computed tomography (CT) for 9 days. Twenty-one days post-transplantation, histopathological analysis and apoptosis assay were performed. ADSC viability and differentiation were not affected by 124I-HIB labeling. In vivo tracking of the 124I-HIB-labeled ADSCs was possible for 9 and 3 days in normal and MI model, respectively. Apoptosis of transplanted cells increased in the MI model compared than that in normal model. We developed a direct labeling agent, 124I-HIB, and first tried to longitudinally monitor transplanted stem cell to MI. This approach may provide new insights on the roles of stem cell monitoring in living bodies for stem cell therapy from pre-clinical studies to clinical trials.
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