黄嘌呤氧化酶
木犀草素
化学
次黄嘌呤
高尿酸血症
尿酸
痛风
黄嘌呤
对接(动物)
圆二色性
生物化学
立体化学
活动站点
结合位点
酶
类黄酮
抗氧化剂
医学
护理部
作者
Jiakai Yan,Guowen Zhang,Yuting Hu,Yue Ma
出处
期刊:Food Chemistry
[Elsevier]
日期:2013-12-01
卷期号:141 (4): 3766-3773
被引量:144
标识
DOI:10.1016/j.foodchem.2013.06.092
摘要
Xanthine oxidase (XO) catalyses hypoxanthine and xanthine to uric acid in human metabolism. Overproduction of uric acid will lead to hyperuricemia and finally cause gout and other diseases. Luteolin is one of the major components of celery and green peppers, its inhibitory activity on XO and their interaction mechanism were evaluated by multispectroscopic methods, coupled with molecular simulation. It was found that luteolin reversibly inhibited XO in a competitive manner with inhibition constant (Ki) value of (2.38 ± 0.05) × 10−6 mol l−1. Luteolin could bind to XO at a single binding site and the binding was driven mainly by hydrophobic interactions. Analysis of synchronous fluorescence and circular dichroism spectra demonstrated that the microenvironment and secondary structure of XO were altered upon interaction with luteolin. The molecular docking results revealed luteolin actually interacted with the primary amino acid residues located within the active site pocket of XO.
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