香兰素
木质素
化学
阿魏酸
生物化学
突变体
食品科学
有机化学
基因
作者
P. D. Sainsbury,Elizabeth M. Hardiman,Masroor Ahmad,Hiroshi Otani,Nicolas Seghezzi,Lindsay D. Eltis,Timothy D. H. Bugg
摘要
The aromatic polymer lignin represents a possible renewable source of aromatic chemicals, if biocatalytic routes for lignin breakdown can be developed. The availability of a genome sequence for Rhodococcus jostii RHA1, a bacterium that breaks down lignin, has allowed the application of a targeted pathway engineering strategy to lignin breakdown to produce vanillin, a valuable food/flavor chemical. A gene deletion strain of R. jostii RHA1 in which the vanillin dehydrogenase gene had been deleted, when grown on minimal medium containing 2.5% wheat straw lignocellulose and 0.05% glucose, was found to accumulate vanillin with yields of up to 96 mg/L after 144 h, together with smaller amounts of ferulic acid and 4-hydroxybenzaldehyde.
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