羊水栓塞
病理
羊水
免疫染色
染色
免疫过氧化物酶
免疫组织化学
单克隆抗体
表位
粘蛋白
肺
H&E染色
医学
抗体
生物
免疫学
胎儿
内科学
怀孕
遗传学
作者
Hiroshi Kobayashi,Hidekazu Ooi,Hiroshi Hayakawa,Tomio Arai,Yoshihisa Matsuda,Kaoru Gotoh,Toshihiko Tarao
标识
DOI:10.1016/s0046-8177(97)90031-9
摘要
This study evaluates whether immunohistochemical staining using antibody TRH-2 is presented as a sensitive method for the histological diagnosis of amniotic fluid embolism (AFE). TKH-2 is the sensitive antibody clearly directed to sialyl Tn, NeuAc α 2–6GaINAc and reacts with meconium- and amniotic fluid-derived mucin-type glycoprotein. Formalin-fixed, paraffin-embedded maternal lung tissue sections were obtained from four cases of patients showing AFE, and four women uninvolved with AFE served as control. Specimens were stained using the streptavidin-biotin-immunoperoxidase method. The results of immunostaining were compared with those of hematoxylin-eosin (H&E) or a conventional Alcian blue stain. Remarkable positive TKH-2 stainings were observed easily within the pulmonary vasculature in patients with AFE syndrome. AFE can be easily missed on H&E sections. Compared with TKH-2 staining, Alcian blue staining also may be insufficient to show intravascular mucin in the maternal lung sections. TKH-2 immunostaining is the sensitive method to detect meconium-and amniotic fluid-derived mucin in the lung sections of patients with AFE syndrome.
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