Gene transfer of human TCR in primary murine T cells is improved by pseudo‐typing with amphotropic and ecotropic envelopes

We set up a quick and versatile method to genetically modify primary murine T cells based on transient production of TCR-positive retroviruses, and show that retroviral gene transfer of a human TCR into primary murine T cells is critically improved by viral pseudo-typing with both MLV-A and MLV-E envelopes.