The orientation of the motor fascicle of the median nerve in the carpal tunnel was investigated in dissections of 50 hands. Topographically, the motor branch was located on the radial-volar aspect of the median nerve in 60% of the hands, the central-volar aspect in 22%, and between these two locations in the remaining 18%. In 56% of the hands, the motor branch passed through a separate distinct fascial tunnel before entering the thenar muscles. Awareness of these patterns will facilitate appropriate surgical management of thenar muscle weakness or wasting associated with the carpal tunnel syndrome.