胞浆
细胞内
核酸
化学
纳米技术
细胞
药物输送
细胞室
小分子
生物物理学
细胞生物学
计算生物学
酶
生物化学
生物
材料科学
作者
Alejandro Méndez‐Ardoy,Irene Lostalé‐Seijo,Javier Montenegro
出处
期刊:ChemBioChem
[Wiley]
日期:2018-11-20
卷期号:20 (4): 488-498
被引量:26
标识
DOI:10.1002/cbic.201800390
摘要
Abstract The internalisation and delivery of active substances into cells is a field of growing interest for chemical biology and therapeutics. As we move from small‐molecule‐based drugs towards bigger cargos, such as antibodies, enzymes, nucleases or nucleic acids, the development of efficient delivery systems becomes critical for their practical application. Different strategies and synthetic carriers have been developed; these include cationic lipids, gold nanoparticles, polymers, cell‐penetrating peptides (CPPs), protein surface modification etc. However, all of these methodologies still present limitations relating to the precise targeting of the different intracellular compartments and, in particular, difficulties in access to the cellular cytosol. Additionally, the precise quantification of the cellular uptake of a compound is not enough to demonstrate delivery and/or functional activity. Therefore, methods to determine cellular distributions of cargos and carriers are of critical importance for identifying the barriers that are blocking the activity. Herein we survey the different techniques that can currently be used to track and to monitor the subcellular localisation of the synthetic compounds that we deliver inside cells.
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