生物
分生组织
等位基因
基因座(遗传学)
基因
巴巴多斯棉
突变体
遗传学
拟南芥
象形文字
植物
栽培
作者
Ryan J. Andres,Viktoriya Coneva,Margaret Frank,John R. Tuttle,Luis Fernando Samayoa,Sang-Won Han,Baljinder Kaur,Linglong Zhu,Hui Fang,Daryl T. Bowman,Marcela Rojas-Pierce,Candace H. Haigler,Don C. Jones,James B. Holland,Daniel H. Chitwood,Vasu Kuraparthy
标识
DOI:10.1073/pnas.1613593114
摘要
Leaf shape varies spectacularly among plants. Leaves are the primary source of photoassimilate in crop plants, and understanding the genetic basis of variation in leaf morphology is critical to improving agricultural productivity. Leaf shape played a unique role in cotton improvement, as breeders have selected for entire and lobed leaf morphs resulting from a single locus, okra (l-D1), which is responsible for the major leaf shapes in cotton. The l-D1 locus is not only of agricultural importance in cotton, but through pioneering chimeric and morphometric studies, it has contributed to fundamental knowledge about leaf development. Here we show that an HD-Zip transcription factor homologous to the LATE MERISTEM IDENTITY1 (LMI1) gene of Arabidopsis is the causal gene underlying the l-D1 locus. The classical okra leaf shape allele has a 133-bp tandem duplication in the promoter, correlated with elevated expression, whereas an 8-bp deletion in the third exon of the presumed wild-type normal allele causes a frame-shifted and truncated coding sequence. Our results indicate that subokra is the ancestral leaf shape of tetraploid cotton that gave rise to the okra allele and that normal is a derived mutant allele that came to predominate and define the leaf shape of cultivated cotton. Virus-induced gene silencing (VIGS) of the LMI1-like gene in an okra variety was sufficient to induce normal leaf formation. The developmental changes in leaves conferred by this gene are associated with a photosynthetic transcriptomic signature, substantiating its use by breeders to produce a superior cotton ideotype.
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