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Transcriptome analysis uncovers salt stress-responsive mechanisms and GmCML48 as a key candidate gene in soybean

转录组 候选基因 生物 植物激素 信号转导 基因家族 基因 遗传学 生物途径 转录因子 下调和上调 基因表达调控 RNA序列 代谢途径 转基因 基因表达 细胞生物学 从头转录组组装 脱落酸 单倍型 转录调控 基因型 折叠变化 表型 基因表达谱
作者
Shuangzhe Li,Yitong Li,Wei Qiang,Yuanrui Zhang,Ming Yuan,Siming Wei,Chang Xu,qingshan chen,Zhenbang Hu,Ying Zhao,Limin Hu
出处
期刊:Plant Stress [Elsevier BV]
卷期号:19: 101231-101231 被引量:1
标识
DOI:10.1016/j.stress.2026.101231
摘要

• Transcriptomic analysis reveals the differences among genotypes and tissues in salt response. • Significant enrichment differences in the plant-pathogen interaction pathway were observed between salt-tolerant variety Q and salt-sensitive variety D. • GmCML4 8 exhibits high expression levels in roots and is potently induced by NaCl, NaHCO₃, ABA, and BR. • GmCML48 improves growth and antioxidant enzyme activities to enhance salt tolerance. • Haplotype analysis identifies Hap_A as the superior salt-tolerant haplotype of GmCML48 . Salt stress severely affects soybean yield and cultivation area. A comprehensive understanding of the molecular mechanisms underlying soybean response to salinity is essential for the development of salt-tolerant cultivars. In this study, transcriptome profiling was employed to compare salt-sensitive (D) and salt-tolerant (Q) variety by analyzing the leaf and root tissues under salt stress. A total of 6,276 and 3,278 differentially expressed genes (DEGs) were identified in the leaves and roots of salt-tolerant variety Q, whereas 9,761 and 11,875 DEGs were detected in salt-sensitive variety D, respectively. The regulatory pathways activated under salt stress exhibited significant differences between genotypes and tissues. The plant hormone signal transduction pathway was strongly activated in both tissues of both cultivars, with key genes in multiple hormone pathways demonstrating substantially higher transcriptional upregulation in the salt-tolerant variety Q. Notably, the activation level of the plant-pathogen interaction pathway differed greatly between varieties, with the GmCML gene family accounting for a major proportion of this pathway. Comparative analysis identified GmCML48 as the gene most strongly induced by NaCl, NaHCO 3 , ABA, and BR treatments. Transgenic overexpression lines displayed significant improvements in growth traits and antioxidant enzyme activities under salt stress, establishing GmCML48 as a key contributor to soybean salt tolerance. Furthermore, Hap_A was identified as a haplotype associated with superior salt tolerance. These findings could advance the understanding of soybean molecular adaptations to saline environments and provide a theoretical basis for targeted breeding of salt-tolerant cultivars.
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