生物
互补DNA
基因
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基因表达
热冲击系数
遗传学
分子克隆
分子生物学
克隆(编程)
转录因子
植物
热休克蛋白
肽序列
热休克蛋白70
程序设计语言
计算机科学
作者
Meng Guo,Yanxu Yin,J.-J. Ji,Biao Ma,Ming‐Hui Lu,Zhen-Hui Gong
出处
期刊:Genetics and Molecular Research
[Genetics and Molecular Research]
日期:2014-01-01
卷期号:13 (1): 1865-1875
被引量:35
标识
DOI:10.4238/2014.march.17.14
摘要
The heat-shock transcription factor (Hsf) gene CaHsfA2 (GenBank accession No. JX402923) was cloned from the Capsicum annuum thermotolerant line R9 by combining the techniques electron cloning and rapid amplification of cDNA ends.The gene, which is 1436 bp in length, had an open reading frame of 1089 bp that encoded 362 amino acids.There was an 831-bp intron between positions 321 and 322 of the cDNA.The deduced amino acid sequence of CaHsfA2 contained the conserved domains of Hsf, including DNA binding domain, adjacent domain with heptad hydrophobic repeats (A/B), activator motifs, nuclear localization signal, and nuclear export signal, and it had the highest E value of hypothesized annotation of HsfA2.CaHsfA2 had the nearest phylogenetic relationship with HsfA2 from Lycopersicon peruvianum and Mimulus guttatus, which was consistent with its botanical classification.After heat-shock treatment at 40°C for 2 h, the expression of CaHsfA2 was observed in different tissues of thermotolerant cultivar R9 and thermosensitive line B6; however, the expression levels of the ©FUNPEC-RP www.funpecrp.com.
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