The composition of the essential oil obtained from the dried flowering aerial parts of Marrubium astracanicum Jacq. (Labiatae) was analyzed by GC and GC-MS. Fifty-nine components have been identified in the essential oil of M. astracanicum. The major constituents of the essential oil were methylcyclopentane (15.5%), thymol (10.6%) and n-heptane (7.4%).