[Objective] To optimize the condition for ultrasonic-microwave synergistic extraction process of total flavones from the water chestnut peel. [Method] The single factor analysis and orthogonal test were designed to confirm the optimum condition for ultrasonic-microwave synergistic extraction process of total flavones from the water chestnut peel. [Result] The results showed that the optimum conditions were as follows: extraction time 25 min,extraction temperature 60 ℃,solid to liquid ratio1∶16 g / ml,ethanol concentration 70%,and finally the extraction rate of total flavones was 2. 652%. [Conclusion] The study can provide theoretical basis for fully utilization of active ingredients of water chestnut peel.