Studies on Tissue Culture System of Malus sieversii

The stem segments of Malus sieversii were used as explants to study the tissue culture.By sterilizing the explants with the common disinfection method on woody plants and comparing the effects with different methods,the results indicate that the best disinfection method was rinsing the explants with 70% alcohol for 15 s,then immersing them in 0.1% HgC12 for 10 min,and finally immersing them in 70% alcohol for 10 s.The best induction medium was modified MS+0.5 mg/L 6-BA+0.1 mg/L NAA,the optical buds multiplication medium was modified MS+0.5 mg/L 6-BA,and the best rooting medium was modified MS+1 mg/L NAA.