Study on inducing hairy roots of Pogostemon cablin Ait. by Agrobacterium rhizogenes

Objective To obtain the best conditions of inducing hairy roots of Pogostemon cablin and establish the system of inducing hairy roots of Pogostemon cablin and provide basis for further study.Methods Co-culture method was used to induce hairy roots of Pogostemon cablin by Agrobacterium rhizogenes ATCC15834 and C58C1 after pre culture,infection,co-culture and sterilization operations.Results Under the conditions of pre culture for 2 days,15 mg / L acetosyringone,infection of 25 min and coculture for 2 days,the induction rate of Agrobacterium rhizogenes ATCC15834 and C58C1 infecting Pogostemon cablin explants were the highest of 83.3% and 80.5%. The PCR amplification results confirmed that the part of T-DNA from Ri plasmid of Agrobacterium rhizogenes was in Pogostemon cablin hairy root genome integration and expression,indicating that Pogostemon cablin hairy roots have been induced successfully. Conclusion The system of inducing hairy roots of Pogostemon cablin were successfully induced and established.