Objective To investigate human papillomavirus(HPV) prevalence and subtypes distribution among women with cervical lesion in Harbin.Methods During September 2009 to November 2010,the HPV types were tested among 1 029 women who were suspected to be suffering from HPV infection or cervical lesion in Harbin.Results The overall positive rate of involved 1 029 women was 31.20%(321/1 029),of which 25.66%(264/1 029) were infected by high-risk types,5.54%(57/1 029) by low-risk types,and 7.09%(73/1 029)by multiple infection.Twenty-one types were detected,of which HPV16(9.72%) was the most common type,followed by HPV53(4.08%).Conclusion Cervical HPV infection has certain regionity.HPV16 and HPV53 is the common type in the HPV infection of suspected to be suffering from HPV infection or cervix lesion in Harbin.