RAPD Molecular Markers Between Jian Carp and Huanghe Carp and Genetic Analysis of Heterosis

RAPD technique was used to study Jian carp, Huanghe carp and their F1 hybrid . With 33 random primers selected, 155 bands were produced and 105 among them were polymorphic. There were two species specific amplification bands produced by primer S18, S472 (1600, 300 bp in size, respectively) which could be used as molecular genetic markers to identify Jian carp from Huanghe carp. Genetic similarity indices (S) for the four populations of Jian carp, Huanghe carp, and their offspring were 0.8240 , 0.7921 , 0.7920 and 0.8569, respectively. The two populations of Huanghe carp and F1 hybrid of Jian carp♀×Huanghe carp♂ maintained the higher degrees of the intrapopulation genetic variation. The offspring of Jian carp♂×Huanghe carp♀ had the smallest degree of genetic variation. The interspecific genetic distances was 0.2233 (Jian carp♀×Huanghe carp♂ to Jian carp), 0.2436 (Jian carp♀×Huanghe carp♂ to Huanghe carp ), 0.1749 (Jian carp♂×Huanghe carp♀ to Jian carp ) , and 0.2026 (Jian carp♂×Huanghe carp♀ to Huanghe carp )respectively. The data revealed that the F1 hybrid inherited more genetic material from Jian carp than Huanghe carp. Analysis of RAPDmarker types and number in the F1 hybrids and their parents was conducted. Heterosis of F1 hybrid were determined using PIT tag system and the genetic mechanism was analysed.