An optical fiber biosenor based on electrochemiluminescence for glucose analysis

An optical fiber biosensor based on luminol electrochemiluminescence(ECL) was developed for determination of glucose. Using carbon paste as the solid support, glucose oxidase (GOD) was bulk immobilized in the carbon paste electrode. The enzymatic reaction electrooxidation of luminol and chemiluminescent reaction occurred simultaneously on the surface of the carbon paste electrode, so the response of the biosensor was very fast. The peak intensity was achieved within only 10 seconds after glucose injection. The linear range extends from 1.0×10 -5 to 2.0×10 -2 mol/L for glucose with detection limit of 6.4×10 -6 mol/L. The biosensor was applied to determination of glucose in soft drinks.