With the pig bone enzymatic hydrolyzate was fermented by lactobacillus.the influence of fermenting microbe and different fermentation temperatures,inoculation volume,fermentation times on fermentation was investigated.Orthogonal experiments were adopted to analyze the effect of each factor in fermentation temperature,inoculates,fermentation times according to single factor experiment.Optimum fermentation conditions were determined as follows:fermentation temperature 30 ℃,inoculation volume 20%,fermentation times 18 h,the PTA-SN and the contents of amion-N reach 0.5764 and 0.041,respectively.