An Investigation of β Cyclodextrin Chiral Mobile Phase Additive in HPLC III The Simplified Calculation and Experimental Verification of the Stability Constants of Enantioselective Inclusion Complexation with β Cyclodextrin

Based on the literature and experimental results, investigation on the effect of β cyclodextrin as mobile phase additive on the retention behaviour of methylphenobarbital enantiomers was carried out with a simplified equation for the calculation of inclusion complex constant The relationship between the inclusion complex constants of β cyclodextrin and solvent strenth was also studied The results indicated that the simplified mathematical model had the advantages of simpler calculation, smaller error and better linearity