Study on the Factors Influencing the Proliferation of Butterfly Orchids Pedicel Buds

[Objective] The research aimed to study the factors influencing the proliferation rate of butterfly orchids(Phalaenopsis spp) pedicel buds.[Method] In flower bud period,the pedicel buds with 2,3,4,5 and 6 cm were selected as the tested materials and they were divided into 1/4,1/2 and 3/4 segments to study the effect of the pedicel bud selecting time,medium formula and dark culture on the proliferation rate of butterfly orchids flower buds.[Result] It was found that 5-6 cm and 3-4 cm of pedicel buds were different on the number of differentiated auxiliary bud,but they had no obvious difference on total proliferation rates.Dark culture could induce the production of adventitious bud better,but it had greater effect on the pedicel buds with 1/4 and 1/2 segments.In the view of the segments of the pedicel buds,3/4 pedicel bud got the best inducing effect.In orthogonal test for screening medium,it was found that KCl and KT could improve the proliferation rate,and NAA had some inhibition on the proliferation of pedicel buds.[Conclusion] The medium screened in the test(1/2 MS + KCl 1.0 g/L + BA 5.0 mg/L + NAA 0.6 mg/L + KT 1.0 mg/L) could make the proliferation rate of butterfly orchids up to 8.5,being 3-4 times of initial proliferation rate in test.