Nanometal Surface Energy Transfer-based Lateral Flow Immunoassay for T2 Toxin Detection

In this study, we incorporated nanometal surface energy transfer (NSET) in lateral flow immunoassay (LFIA) and explored the relationship between fluorescence quenching efficiency and detection sensitivity to improve sensitivity of NSET-LFIA system. We developed nine gold nanoparticles (GNPs) with absorption spectrum in the range of 520-605 nm as acceptors and quantum dot microspheres (QDMs) with emission spectrum of 530, 570, and 610 nm as donors. By analyzing the overlap integral area, fluorescence quenching efficiency, and detection sensitivity of 27 donor-acceptor pairs, we observed that the larger overlap integral area led to higher fluorescence quenching efficiency and detection sensitivity. A maximum fluorescence quenching efficiency of 91.0% was obtained from the combination of GNPs at 605 nm and QDMs at 610 nm, achieving the highest detection sensitivity. We developed NSET-LFIA for the detection of T2 toxin with a limit of detection of 0.04 ng/mL, which was 10-times higher than that obtained via conventional GNP-LFIA. NSET-LFIA represents a versatile, ultrasensitive and valuable screening tool for small molecules in real samples.