树突棘
体内
神经科学
生物
神经可塑性
皮质(解剖学)
可塑性
细胞生物学
脊柱(分子生物学)
物理
遗传学
海马结构
热力学
作者
Albert Hiu Ka Fok,Yuhua Huang,Beth Wing Lam So,Qiang Zheng,Christin Tse,Xiaoyang Li,Kenneth K. Y. Wong,Jiandong Huang,Kwok-On Lai,Cora Sau Wan Lai
出处
期刊:Cell Reports
[Elsevier]
日期:2024-03-01
卷期号:43 (3): 113906-113906
标识
DOI:10.1016/j.celrep.2024.113906
摘要
Summary
Kinesin 1 (KIF5) is one major type of motor protein in neurons, but its members' function in the intact brain remains less studied. Using in vivo two-photon imaging, we find that conditional knockout of Kif5b (KIF5B cKO) in CaMKIIα-Cre-expressing neurons shows heightened turnover and lower stability of dendritic spines in layer 2/3 pyramidal neurons with reduced spine postsynaptic density protein 95 acquisition in the mouse cortex. Furthermore, the RNA-binding protein fragile X mental retardation protein (FMRP) is translocated to the proximity of newly formed spines several hours before the spine formation events in vivo in control mice, but this preceding transport of FMRP is abolished in KIF5B cKO mice. We further find that FMRP is localized closer to newly formed spines after fear extinction, but this learning-dependent localization is disrupted in KIF5B cKO mice. Our findings provide the crucial in vivo evidence that KIF5B is involved in the dendritic targeting of synaptic proteins that underlies dendritic spine plasticity.
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