瘤胃
细菌
纤维素酶
食品科学
酵母抽提物
酵母
16S核糖体RNA
基质(水族馆)
酶
化学
生物
发酵
制浆造纸工业
生物技术
生物化学
生态学
遗传学
工程类
作者
Mustafa Özkan Baltacı,Mehmet Akif Omeroglu,Seyda Albayrak,Gülşah Adıgüzel,Ahmet Adıgüzel
标识
DOI:10.1590/0001-3765202220220151
摘要
Millions of tons of agricultural waste are produced globally every year. A practical solution to this global problem is to convert this waste into value-added products. In this study, endoglucanase enzyme production was carried out by using waste melon peels as a carbon source. To use this important resource, its stubborn structure must be broken down. Rumen bacteria are regarded as unique for this job. Therefore, firstly endoglucanase producing rumen bacteria was isolated and the bacteria with the best activity (OB24) were identified by molecular methods (16S rRNA gene squencing). As a result of the sequence analysis, it was determined that isolate belonged to Exiguobacterium mexicanum. Then, by optimizing the culture conditions, the enzyme production potential was increased. The optimal conditions were determined as 50 g/L MPP, 2g/L yeast extract, 60 h incubation time, pH: 6.0, and 40°C temperature. Under optimized conditions the enzyme activity increased approximately 3.8-fold.
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