皂甙
齐墩果酸
化学
水解
藜藜
成分
色谱法
乙醇
酸水解
高效液相色谱法
食品科学
萃取(化学)
生物化学
医学
替代医学
病理
作者
Daniel Mejía-Valdez,Marilena Antunes‐Ricardo,Mariana Martínez-Ávila,Daniel Guajardo‐Flores
标识
DOI:10.1016/j.foodchem.2024.139254
摘要
The study investigated Chenopodium berlandieri to analyze its oleanolic acid (OA) content. Response surface methodology with central composite design was used to improve saponin extraction, varying temperature, ethanol, and sample-to-solvent ratio. Best conditions (65 °C, 50% ethanol, 1:10 ratio) yielded 53.45 ± 0.63 mg/g of extract from Huauzontle seeds. Temperature linearly impacted extract yield, while temperature and ethanol influenced total saponin content. Hydrolyzing saponin-rich extracts produced OA-rich extracts. Characterization via HPLC-ELSD and LC-MS identified OA4 as the most concentrated OA saponin (5.54 ± 0.16 mg/g). OA alone reached 2.02 ± 0.12 mg/g. Acid hydrolysis increased OA content by up to 3.27×, highlighting the potential of hydrolyzed Huauzontle extracts as a natural ingredient for various industries due to enhanced OA content.
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