脂解
细胞内
巨噬细胞
欧米茄
荧光
化学
生物化学
生物物理学
细胞生物学
生物
脂肪组织
物理
体外
量子力学
作者
H. S. Tang,Zuguo Liu,Guangmei Han,Junlong Geng,Bianhua Liu,Rui-Long Zhang,Zhongping Zhang
标识
DOI:10.1073/pnas.2321255121
摘要
Omega-3 polyunsaturated fatty acids (PUFA) found primarily in fish oil have been a popular supplement for cardiovascular health because they can substantially reduce circulating triglyceride levels in the bloodstream to prevent atherosclerosis. Beyond this established extracellular activity, here, we report a mode of action of PUFA, regulating intracellular triglyceride metabolism and lipid droplet (LD) dynamics. Real-time imaging of the subtle and highly dynamic changes of intracellular lipid metabolism was enabled by a fluorescence lifetime probe that addressed the limitations of intensity-based fluorescence quantifications. Surprisingly, we found that among omega-3 PUFA, only docosahexaenoic acid (DHA) promoted the lipolysis in LDs and reduced the overall fat content by approximately 50%, and consequently helped suppress macrophage differentiation into foam cells, one of the early steps responsible for atherosclerosis. Eicosapentaenoic acid, another omega-3 FA in fish oil, however, counteracted the beneficial effects of DHA on lipolysis promotion and cell foaming prevention. These in vitro findings warrant future validation in vivo.
科研通智能强力驱动
Strongly Powered by AbleSci AI