生物
类有机物
肠上皮
组织谷氨酰胺转胺酶
去酰胺
免疫学
肠粘膜
免疫系统
上皮
疾病
细胞
面筋
发病机制
细胞生物学
病理
遗传学
酶
医学
内科学
食品科学
生物化学
作者
V. A. Dotsenko,Amir‐Babak Sioofy‐Khojine,Heikki Hyöty,Keijo Viiri
标识
DOI:10.1016/bs.mcb.2023.01.008
摘要
Celiac disease pathogenesis, in addition to immune cell component, encompasses pathogenic events also in the duodenal epithelium. In celiac disease patients, exposure to dietary gluten induces drastic changes in epithelial differentiation and elicit cellular response to inflammatory cytokines. The autoantigen in celiac disease, transglutaminase 2 (TG2) enzyme, has been also suggested to play its pathogenic gliadin deamidation event in the intestinal epithelium. Therefore in vitro epithelial cell-line models have been exploited in the past to study these pathogenic mechanisms, but they are hampered by their simplistic nature lacking proper cell-type composition and intestinal environ. Moreover, these cell models harbor many cancer-related mutations in tumor suppressor genes making them unsuitable for studying cell differentiation. Intestinal organoids provide a near-native epithelial cell model to study pathogenic agents and mechanisms related to celiac disease. Here we describe protocols to initiate and maintain celiac patient-derived organoid cultures and how to grow them in alternative ways allowing their exploitation in different kind of experiments.
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